Diagenode

Applications

  • Epigenetics DNA Methylation

    Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication ... Learn more

    Bisulfite conversion

    Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine D... Learn more

    Methylated DNA immunoprecipitation

    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is pr... Learn more

    Methylbinding domain protein

    The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to... Learn more

  • Epigenetics Chromatin Analysis

    Epigenetics research addresses alterations to chromatin that propagate different patterns of transcription or gene expression and silencing. The major components of chromatin are DNA and histone proteins. Two copies of each histone core protein (H2A, H2B, H3 & H4) are assembled into an octamer, wrapped around by DNA to form a nucleosome core. The nucleosome controls the accessibility of DNA to the transcription machinery and chromatin remodelling factors. Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluabl... Learn more

    ChIP-qPCR

    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus o... Learn more

    ChIP-seq

    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life sci... Learn more

    Chromatin shearing

    The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bioruptor® uses state-of-the-art ultrasound ACT (Adaptive Cavitation ... Learn more

  • Next Generation Sequencing

    DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 kb to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA library prep and FFPE nucleic acid extraction Shear anywhere from 2 kb – 75 kb Perfect for mate pair library prep and long fragment DNA sequencing Shear 20 or 50 µl with this light desktop device 2. Choose your optimized library preparation kit 3. Choose library prep automation for unmatched data reproducibility Low input of 50 pg... Learn more

    DNA/RNA shearing

    In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to... Learn more

    FFPE DNA extraction

    Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high y... Learn more

    Library preparation

    Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphoryl... Learn more

    RNA extraction

    Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diageno... Learn more

  • Protein Analysis

    Proteins play a critical role in virtually all cell processes including metabolism, structure, growth, and repair. Understanding protein function, behavior, structure, and their importance are key aspects of understanding disease and therapeutics. Experimental analysis of proteins typically involves expression and purification or the direct extraction of proteins from cells or tissues for further downstream analysis such as ligand binding assays, mass spectrometry, or protein sequencing. Other protein analysis methods include detection methods such as BCA assays and Western blots or methods to understand protein-to-protein in... Learn more

    Protein extraction

    Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purificati... Learn more

    Western blot analysis

    We have what you need for a successful Western blot! Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly. For a successful Western blot we offer: Loa... Learn more

  • Antibody Applications

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    Protein array

    Protein array Learn more

    DB

    Dot blotting Learn more

    ELISA

    Enzyme-linked immunosorbent assay. Learn more

    GSA

    Gel Supershift Learn more

    IC

    Immunochemistry Learn more

    IF

    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°... Learn more

    IHC

    Immunohistochemistry Learn more

    IP

    Immunoprecipitation Learn more

    Peptide array

    Peptide array Learn more

    siRNA Knockdown

    Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-hist... Learn more

    WB

    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Loading control, MW marker visualization. Check our selection of antibod... Learn more

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