Nucleophosmin EMSA Kit

Size: 100 reactions

Storage: -70°C

Key features: Purified NPM is in a multimer conformation and highly active in interacting with RNA. In a standard reaction (10 ul), NPM (50 ng) forms stable complexes with virtually all RNA substrates (1 nM) provided. However, the optimal concentration of NPM1 should be emperically determined for each RNA substrate of researcher's interest. The following reagents will be provided:

• NPM: 5 ug (0.1 mg/ml).
• 10x Reaction buffer (0.25 ml): 0.2 M Hepes-NaOH, pH 7.4, 1 M NaCl, 10 mM DTT, and 2 mg/ml BSA.
• 5x Loading buffer (0.5 ml): 0.1 M Tris-HCl, pH 7.4, 5 mM EDTA, 0.05% NP-40, 0.01% bromophenol blue, 0.01% xylene cyanol, and 50% glycerol
• Protein dilution buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 50 mM NaCl, 0.1 mM EDTA, 0.5 mM PMSF, 0.2 mg/ml bovine serum albumin, 0.05% Noniodit P-40, a cocktail of protease inhibitors, and 20% glycerol.

Product reference: kva-BS-001

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