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MethylEasy™ Kits
MethylEasy™ Xceed
Detect every Methylated Cytosine of your Target sequences!
> Quick & simple: no DNA pre-treatment and no precipitation step
> Maximized efficiency of DNA denaturation, bisulphite conversion and amplification
> Flexible and best sensivity mapping of methylated cytosines
| Original MethylEasy™ |
MethylEasy™Xceed |
| Min 1 ng of DNA |
Min 50 pg of DNA |
| Denature DNA with NaOH and incubate for 15 minutes |
Idem |
| Add conversion reagents and incubate for 4-16 hours |
Add conversion reagents and incubate for 45 minutes |
| Precipitate and wash the DNA, 45 to 75 minutes |
Purify the DNA via column for 10 minutes |
| Resuspend the DNA and desulphonate for 30 mins to 1 hour |
Desulphonate for 20 minutes |
| Full conversion 6 to 18 hours total time |
Full conversion 90 minutes total time |
Take a Leap Forward in Methylation Detection
• More sensitive
Able to start with as low as 50pg of human DNA for ultra-fine detection of DNA methylation from just a few cells latter flexible for removal
• More Reactions
40 reactions total volume provided, for higher throughput needs and great new value per kit
• Internal Controls
Ensure unequivocal confidence in results with reliable reaction confirmation. Also enabling fully informed HGS support during problem solving.
• NEW: Useful in the Broadest Applications
Where hypersensitivity is critical, e.g. stem cell uniformity, fine needle tumour biopsies and where material needs to be highly preserved post-conversion, enabling the interrogation of multiple loci after only one conversion step.
• NEW: Flexible incubation options
Either fully ready to be incubated in PCR reaction tubes; or conventional oven or incubator instrumentation.
• Markedly Faster Reactions than anything else on the market
Less than 90 mins total time from wild type to fully modified DNA.
• Easier user handling
Simple column-based protocol, maintaining powerful sensitivity
• Excellent DNA Preservation
Maintains world-renown “loss-less” DNA preservation qualities previously observed in HGS’ existing MethylEasy™ kit
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