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In Vitro Sumoylation Kit

Size: 50 reactions

Key features: In addition to highly active human SAE1/SAE2 (50 units), this assay kit contains the following reagents.

  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 50 mM MgCl2, and 20 mM ATP, 0.5 mM PMSF, and 1 mg/ml BSA.
  • SUMO-1 (50 ul, 1 mg/ml)
  • GST-UBC9 (50 ul, 0.4 mg/ml)
  • Control peptide (Gst-IkBa, 50 ul, 0.5 mg/ml) *an other version of the kit is available with Control peptide (Flag/GST-RHA 137, 50 ul, 0.5mg/ml)
  • 5x SDS sample buffer
Product reference: kva-SA-001 product reference top