A powerful duo for ChIP-seq
using just 10,000 cells

True MicroChIP Kit and MicroPlex Library Preparation Kit

  • Revolutionary: Only 10,000 cells needed for complete ChIP-seq procedure
  • Reliable and accurate sequencing library preparation from just picogram inputs
  • Validated on studies for histone marks and with the IP-Star® Compact Automated Platform

>> How it works: See complete workflow

Reliable detection of enrichments in ChIP-seq

ChIP was peformed with H3K4me3 antibody, 17 pg of DNA (subsequently amplified), ChIP'd from 10,000 cells and 35 pg of control DNA (subsequently amplified), ChIP'd from 100,000 cells. The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina platform with the True MicroAmplification kit. The library was analyzed on an Illumina Genome Analyzer. Cluster generation and sequencing were performed using manufacturer instructions.

Perfect match with ChIP-seq reference dataset

 

  Matched by Broad Institute dataset
  Unmatched by Broad Institute dataset

We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

 

High efficiency ChIP on 10,000 cells

ChIP efficiency on 10 000 cells. ChIP was performed on human Hela cells using the Diagenode antibodies H3K4me3 (Cat No. pAb-003-050), H3K27ac (pAb-174-050), H3K9me3 (pAb-056-050) and H3K27me3 (pAb-069-050). Sheared chromatin from 10 000 cells and 0.1 µg (H3K27ac), 0.25 µg (H3K4me3 and H3K27me3) or 0.5 µg (H3K9me3) of the antibody were used per IP. Corresponding amount of IgG was used as control. Quantitative PCR was performed with primers for corresponding positive and negative loci. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

 

MicroPlex Library Preparation kit x12 contains ThruPLEX technology developed and manufactured by Rubicon Genomics, Inc.,Ann Arbor, Michigan, USA and covered by US Patent 7,803,550; EP1924704; and US and international patents pending.