Increase sensitivity using Auto MeDIP kit and SX-8G IP-Star^® automated systems

Gene 1: Differencial methylation only detected by the IP-Star^®

Gene 2: Noise is not detected by the IP-Star^®

Methylation patterns were analyzed using MeDIP-on-chip in mouse wildtype and mutant cell lines. The left two panels show the differential methylation patterns of two genes (gene 1 and gene 2) using a manual MeDIP protocol. The right two panels show the differential methylation patterns of the same genes using the Auto MeDIP kit and the IP-Star^® Automated System. The red line corresponds to the transcription start site of the gene. The Y axis corresponds to the differential methylation level of an oligonucleotide probe of a wildtype versus a mutant mouse cell line. Positive values correspond to hypermethylation in the mutant cell line, negative values to hypomethylation in the mutant cell line. X axis represents the nucleotide position. The microarrays used are NimbleGen 385K Mouse promoter and CpG island arrays. All findings were validated by quantitative pyrosequencing of bisulfite-treated DNA.