Generating high-resolution ChIP-Seq profiles using the SX-8G IP-Star^® Platform

Auto-ChIP assays were performed on primary human CD4 and CD8 T cells isolated by negative magnetic separation of peripheral blood mononuclear cells. Each Auto-ChIP sample was performed using Diagenode's Auto-Histone ChIP-seq kit and contained 1 µg of input chromatin. Illumina Genome Analyzer libraries were prepared and the samples were sequenced at the DNA Technologies Core at UC Davis.