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Expertise

IP Automation
Sonication
- More about
DNA Methylation
Antibodies
- Antibody production
Chromatin Function
Protocol Optimization

Expertise | Protocol optimization

Protocol optimization

Let us talk about your personal ChIP, MeDIP and MBD protocol!

ChIP
MeDIP
MBD

Automated ChIP assay

To transfer your unique ChIP protocol to the SX-8G IP-Star, we need some information about your assay.
Please fill out the form, and Diagenode's R&D team will contact you as soon as possible.

What are the advantages of your ChIP protocol? Why have you choosen this specific ChIP protocol? Have you ever performed ChIP using magnetic beads? If yes, can you comment about your experience?

CELL LYSIS

Amount cells / IP

Fixation

Formaldehyde concentration

Time

Stop fixation

Nuclei isolation

CHROMATIN SHEARING

Shearing conditions

Sonicator type (company)

SDS concentration

Settings for shearing

Shearing volumes

Amount of cells

Buffer composition

IMMUNOPRECIPITATION

Beads

Bead type (company)
(agarose, magnetic,...)

Bead volume / IP

Are the beads blocked (reagent)?

Total reaction volume

Alternative precipitation
methods (e.g. Staph A)

Incubation time

1) Chromatin + antibody

2) Chromatin-ab + beads complex

IP buffer composition

IP reaction volume

IP SDS concentration

Antibody amount and volume

Type of protein you target (Histone modifications, transcription factor)

Ratio Ab / chromatin / beads

IP washes

Number of washes

Volume used per wash

Wash buffers composition

Elution

Volume per elution

Buffer composition

DNA PURIFICATION

Method

Final volume

DOWNSTREAM APPLICATIONS (qPCR, sequencing, ...)

  qPCR
  sequencing
  amplification
  microarray
  others

The following fields are required :

Institute / Company :

Last name * :

First name * :

Address * :

City * :

Postcode :

Phone * :

Fax :

E-mail * :

* = obligatory fields

Automated MeDIP assay

To transfer your personal MeDIP protocol to the SX-8G IP-Star, we need some informations about your assay.
Please fill out the form and Diagenode's R&D team will contact you as soon as possible.

GENOMIC DNA EXTRACTION

DNA origin (cell type, tissues, ..)

DNA purification protocol

DNA concentration

DNA SHEARING

Shearing conditions

Sonicator type (company)

Settings for shearing

Shearing volumes

DNA concentration

Buffer composition

Type of tubes

IMMUNOPRECIPITATION

Beads

Bead type (company)

Bead volume / cells

Are the beads blocked (reagent)?

Incubation time

1) Beads + antibody

2) DNA + Ab - beads

DNA amount / IP

2) DNA + Ab - beads

IP reaction volume

Antibody amount and volume

Ratio Ab / DNA / beads

IP washes

Number of washes

Volume used per wash

Wash buffers composition

Elution

Volume per elution

Buffer composition

PURIFICATION

Method

Final volume

Final DNA concentration

The following fields are required :

Institute / Company :

Last name * :

First name * :

Address * :

City * :

Postcode :

Phone * :

Fax :

E-mail * :

* = obligatory fields

Automated MBD assay

To transfer your unique MBD protocol to the SX-8G IP-Star, we need some information about your assay.
Please fill out the form and Diagenode's R&D team will contact you as soon as possible.

GENOMIC DNA EXTRACTION

Amount cells

DNA purification protocol

DNA concentration

DNA SHEARING

Shearing conditions

Sonicator type (company)

Settings for shearing

Shearing volumes

DNA concentration

Buffer composition

Type of tubes

DNA CAPTURE

Beads

Bead type (company)

Bead volume / cells

MBD protein

Type of MBD protein (MBD1, MBD2, MeCP2…)

Amount of MBD Protein/reaction

Capture reaction

MBD-DNA incubation time

DNA amount / reaction

Capture buffer composition

Capture reaction volume

Ratio MBD protein / DNA / beads

washes

Number of washes

Volume used per wash

Wash buffers composition

Elution

Number of elution fractions

Volume per elution

Salt concentration of your Elution fractions

PURIFICATION

Method

Final volume

Final DNA concentration

The following fields are required :

Institute / Company :

Last name * :

First name * :

Address * :

City * :

Postcode :

Phone * :

Fax :

E-mail * :

* = obligatory fields