ChIP-seq has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit and the Diagenode ChIP-seq-grade H3K4me3 antibody.
Image A shows a region of chromosome 12 with high similarity of read distribution between the Diagenode data and the data from the Broad Institute.
Image B shows a comparison between the ChIP-seq dataset from this experiment and a reference dataset from the Broad Institute. We observed a perfect match between the top 40% of Diagenode peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.