Genomic library protocol
New protocol for genomic library prep on the IP-Star® developed in
ChIP-seq grade antibodies
Check out the most validated antibodies on the market for Epigenetics
High reproducibility & flexibility to produce
high quality ChIP-seq data
The IP-Star® Automated System delivers high quality ChIP-seq data
H3K4me3 ChIP-seq results obtained (1) with the Diagenode IP-Star Compact® Automated System and (2) manually.
ChIP was performed on sheared chromatin from 2 million leukemia cells using 2 μg of Diagenode's H3K4me3 antibody and analyzed on an Illumina Genome Analyzer. The figure shows the peak distribution along the GAPDH gene. These results clearly show high similarity of peaks distribution. Data obtained by the IP-Star® showed high correlation with manual ChIP results.
Decrease the amount of chromatin needed for your experiments
Select the best antibodies for your experiments
Diagenode is dedicated to characterize every batch of antibody using established QC procedures, there by maximizing the reproducibility of our antibodies between batches.
Figure 1. Four different batches of H3K4me3 are tested in the same ChIP experiment. Samples are analyzed by qPCR using GAPDH promoter (positive locus) and Myoglobin exon 2 (negative locus) primer pairs.