• Simultaneous sonication of 12 samples - short sample processing time
• Easy-to-use
• Cost effective solution

Figure 1. Comparison of processing time (minutes) per sample for different fragment sizes (150 bp, 200 bp, 500 bp, 1000 bp). Processing time includes hands-on work and sonication time.

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Figure 2. Genomic human DNA has been sheared using the Bioruptor® NGS following validated protocols to obtain fragments from 60-1500 bp. All samples were analysed on Bioanalyzer 2100 using DNA High Sensitivity chip.

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Figure 3. Six genomic human DNA samples were sheared using the Bioruptor® NGS for 15 minutes. All samples were analysed on a Bioanalyzer 2100 using DNA High Sensitivity chip.

Figure 4. Total RNA shearing of epidermal carcinoma human total RNA (Ambion). Lane 1: unsheared RNA; Lane 2 & 3: 5 min of 30 sec. on/30 sec. off sonication time; Lane 4 & 5: 10 min of 30 sec. on/30 sec. off sonication time; Lane 6 & 7: 15 min of 30 sec. on/30 sec. off sonication time.

Diagenode and BGI collaboration

BGI and Diagenode SA Collaborate to Implement Standard Methods in Epigenetics and Next Generation Sequencing Sample Preparation.

Beijing, China and Brussels, Belgium, 14 July 2011 - BGI and Diagenode SA, announced today that they will enter into a close collaboration for the implementation of standard methods in epigenetics and next generation sequencing sample preparation. Diagenode will provide BGI with early access to its new developments in chromatin or DNA methylation analysis methods and tools. Diagenode aims to increase the consistency and precision of enrichment assays, such as ChIP, MeDIP, MBD capture, bisulfite conversion, and DNA fragmentation, prior to next generation sequencing.

"Consistency is extremely important, not only for saving reagents and precious samples, but also for creating universal high standards in the emerging field of epigenetics." Said Didier Allaer, CEO of Diagenode. "We are very proud to be partnering with BGI in these domains."

"We have been successfully using Diagenode's Bioruptors® and reagents for epigenetics, and in exon sequencing, for years now and have implemented them in all of our facilities across China. We are delighted to be able to expand our collaboration with Diagenode, whose reliability and creativity for improving consistency is proven", said Jihua Sun, Lead of Epigenetics Sequencing Platform and Renhua Wu, Lead of Exon Sequencing Platform of BGI-Shenzhen.