Rat TSH2B Primer Pairs (Methylated positive control for MeDIP)

Description
The primer pair cat # pp-1043-050, -500 is specific to a CpG region of the TSH2B gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).

Application: The region amplified with TSH2B primer pair corresponds to genomic locus which is highly methylated in all somatic cells. The primers can be used as a positive control to amplify DNA isolated by Methylated DNA immunoprecipitation (MeDIP) or Methylated DNA Capture (meDNA capture) (Figure 3). In Chromatin Immunoprecipitation (ChIP) assay, the primers can be used as a control representing repressive chromatin.

Specificity
Rat: positive
Other species: not tested

Lot #: 001

Concentration: 10 µM

Expected PCR product size: 99 base pairs (bp)

Amplified locus: chr17:48290238-48290336

Format
In solution in MiliQ water at the concentration of 10 µM (each oligonucleotide primer is at the final concentration of 5 µM).

Storage
For long storage, store at -20°C. Avoid multiple freeze-thaw cycles.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.