Rat GAPDH Primer Pairs (Unmethylated negative control for MeDIP)

Description
The primer pair cat # pp-1046-050, -500 is specific to a promoter region of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene from rat. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).

Application: The region amplified with GAPDH primer pair corresponds to a region of active chromatin. The primers can be used as a negative control to amplify DNA isolated by Methylated DNA immunoprecipitation (MeDIP) or Methylated DNA Capture (meDNA capture) (Figure 3). In Chromatin Immunoprecipitation (ChIP) assay, the primers can be used as a control representing active chromatin.

Specificity
Rat: positive
Other species: not tested

Lot #: 001

Concentration: 10 µM

Expected PCR product size: 66 base pairs (bp)

Amplified locus: chr4:161285768-161285833

Format
In solution in MiliQ water at the concentration of 10 µM (each oligonucleotide primer is at the final concentration of 5 µM).

Storage
For long storage, store at -20°C. Avoid multiple freeze-thaw cycles.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.