Human LMNA promoter Primer Pairs

Description
The primer pair cat#: pp-1011 (-050, -500) is specific to a DNA region in the human Lamin A (LMNA) gene promoter as shown in Figure 1 [1]. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).

Application
The region amplified with GAPDH primer pair corresponds to a region of active chromatin. The primers can be used as a negative control to amplify DNA isolated by Methylated DNA immunoprecipitation (MeDIP) or Methylated DNA Capture (meDNA capture) (Figure 3). In Chromatin Immunoprecipitation (ChIP) assay, the primers can be used as a control representing active chromatin.

Specificity
Human: positive
Other species: not tested

Lot #: 001

Concentration: 10 µM

Expected PCR product size: 120 base pairs (bp)

Format
In solution in MilliQ water at the concentration of 10 µM (each oligonucleotide primer is at the final concentration of 5 µM).

Storage
For long storage, store at -20°C. Avoid multiple freeze-thaw cycles.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.