Hydroxymethyl dCTP

Full name: 5-hydroxymethyl-2'-deoxycytidine-5'-triphosphate (Lithium Salt)

Formula: C₁₀H₁₄Li₄N₃O₁₄P₃

Molecular Weight: 520.9 g/mol

Lambda max: 275 nm

Description
- hmdCTP is supplied as 100 mM aqueous solution titrated to pH 7.5
- > 99% pure by spectral and HPLC analysis
- hmdCTP can be enzymatically incorporated into DNA with MethylTaq DNA polymerase (Cat. No. AF-103-0250)
- Hyrdoxymethylated substrates can be ligated by standard ligases
- Free of endo-, exodeoxyribonuclease, phosphatise and nicking activities

- Synthesis of hydroxymethylated DNA (e.g. using MethylTaq DNA polymerase, Cat. No. AF-102-0300) [page produit]

- Site-directed mutagenesis

- Substitution of dCTP in a wide variety of molecular biology assays

- Structural and activity studies of the restriction/modification systems of different organisms

- Methylation studies (e.g. Immunoprecipitation using a 5-hydroxymethylcytosine antibody - hMeDIP)