DNA precipitant

Description
This IP grade product is used for the precipitation of DNA after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DNA IP). It is included in our Transcription ChIP KIT (cat# kch-redTBP-012), in our Histone ChIP KIT (cat# kch-orgHIS-012) and in our Methylated DNA Immunoprecipitation KIT - MeDIP (cat# mc-green-003). It is now separately available. Following IP and washes, the immunoprecipitated (IP’d) material is submitted to Phenol/Chloroform/Isomanyl alcohol (PCI) extraction. Then, to the aqueous phase obtained after PCI extraction of the IP’d material, our DNA precipitant is added with IP grade DNA co-precipitant (cat# kch-216-100) and icecold 96% ethanol. For more details about these kits, see instruction manual on our web site (www.diagenode.com).

Lot #: D003

Concentration: 3 M solution

Format
Supplied as a 3 M stock solution (pH 5.2). 50 μl allow to precipitate IP’d DNA from a volume of 500 μl.

Storage
The product is stable for at least 2 years when stored at 4°C.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.