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Chromatin Assembly Kit
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Diagenode’s Chromatin Assembly kit is designed to perform in vitro Chromatin Assembly and analysis. The assembled chromatin is then submitted to purification, and subsequent in vitro tests.
|Chromatin Assembly Kit||C01030010 (ca-vitro-003)||20 rxns||CONTACT US|
- Reliable & consistent in vitro chromatin reconstitution
- Novel Step-by-Step Method
- 20 Chromatin Assembly (C.A.) reactions / kit, each involving 2 μg of DNA
The structure of cellular chromatin is capable of undergoing rapid changes to respond to themetabolic requirements of the cell. These changes have a direct impact on gene expression and therefore, the chromatin context must be considered when biochemical reactions that involve DNA are studied. DNA-binding factors have evolved with chromatin. It is therefore more desirable to study the molecular mechanisms of DNA-directed processes with chromatin than with naked DNA templates (Lusser and Kadonaga, 2004). To this end, it is necessary to reconstitute DNA and histones into chromatin.
 Loyola A. and Reinberg D. (2003b) Methods 31:96-103