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MagBisulfite kit
Description
Diagenode's MagBisulfite kit is the only bisulfite conversion kit that contains an optimized DNA purification step based on the use of magnetic beads (IPure technology), which results in high DNA recovery which is compatible for all down-stream applications used for analysis of bisulfite-converted DNA.
| MagBisulfite kit x24 | AF-106-0024 | 24 rxns | 265 $ |  |
Description
Bisulfite conversion is a method to profile DNA methylation at single nucleotide resolution. Sodium bisulfite is used to convert cytosine residues to uracil residues in single-stranded DNA, while 5-methylcytidine remains non-reactive. Our optimized reagents ensure complete conversion and minimize DNA degradation during the treatment. The converted DNA is then desulphonated and purified using IPure magnetic beads. The use of IPure magnetic beads results in high DNA recovery which is compatible for all down-stream applications used for analysis of bisulfite-converted DNA.
Bisulfite conversion workflow
Figure 1: Bisulfite-mediated conversion of cytosine. Bisulfite conversion is performed under acidic conditions and preferentially deaminates cytosine in a nucleophilic attack whilst the methyl group on 5-methylcytosine is protecting the amino group from the deamination. Step 1: Sulphonation; Step 2: deamination; Step 3: desulphonation.
Highest recovery obtained with IPure magnetic beads
Figure 2: Comparison of DNA recovery using the MagBisulfite kit and kits from competitor Q and Z. CTS is a higly methylated region in MCF7 cells.
Figure 3: Human Genomic DNA was converted with Diagenode Bisulfite Conversion kit and purified with IPure Magnetic beads, columns-based purification, GlassMilk or ultrafiltration with Microcon centrifugal filters. IPure magnetic beads purification results in better DNA recovery after bisulfite conversion.
High conversion rate (> 99%)
Figure 4: Methylation profiling by bisulfite sequencing. 1 μg of human genomic DNA from MCF7 cells was converted with Diagenode Bisulfite conversion kit and sequenced to analyze the methylation status of 3 genomic loci. A: CTS region is higly methylated in MCF7 cells. B: claudin region is medium methylated region. C: gapdh is a unmethylated region.
Storage
Store kit components under the conditions indicated on the labels.
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Bisulfite conversion
