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Comparison of commercially available MeDIP kits

“…We observed that the MagMeDIP kit (Diagenode) had the highest yield…and the DNA was successfully enriched from a 1:4 to 10:1 ratio…”

H3K27me3 and H3K4me3

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hMeDIP kit

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Description

This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcytosine (5-hmC) available in the market. It includes control sequences (5-hmC, 5-mC and unmodified cytosine DNA standards) and control primer pairs (internal and external controls), to assess the effiency of your immunoprecipitation.

hMeDIP kit x16 (monoclonal mouse antibody)	AF-110-0016	16 rxns	495 $
hMeDIP kit x16 (monoclonal rat antibody)	AF-104-0016	16 rxns	495 $
hMeDIP kit x16 (polyclonal rabbit antibody)	AF-111-0016	16 rxns	495 $

Characteristics
Applications
Data sheets
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• Immunoprecipiation performed with monoclonal antibody against 5-hmC

• Including internal & external controls for QC of each reaction
> Mouse primers against Sf1 (hydroxymethylated gene in mouse)
> 5-hmC, 5-mC and unmethylated DNA

• Complete magnetic bead based kit

• All in one tube