5-hmC, 5-mC & cytosine DNA standard pack for hMeDIP

Description
The 5-hmC, 5-mC & cytosine DNA standard pack includes 3 hydroxymethylated, methylated and unmethylated DNA standards and their corresponding PCR primer pairs.
The hydroxymethylated DNA standard is a linear dsDNA that has been produced by PCR using the Diagenode’s MethylTaq DNA polymerase (Cat. No. AF-103-0250) and Diagenode’s hydroxymethyl dCTPs (Cat. No. AF-102-0300) replacing normal cytosine nucleotides. The methylated and unmethylated standards have been produced using synthetic oligonucleotides. For the methylated standard, the normal cytosine nucleotides have been replaced by methylcytosine nucleotides. None of these standard show significant homology with human, mouse or rat genomic sequences.
The size of the amplicons produced with the provided primer pairs are:
- Unmethylated control: 69 bp
- Methylated control: 65 bp
- Hydroxymethylated control: 84 bp

Applications
Ideal control DNA for hMeDIP using the 5-hydroxymethylcytosine antibodies included in the hMeDIP (Cat. No. AF-104-0016).

Storage
Store at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, and to prevent cross-contamination. Aliquoting is recommended.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

hMeDIP