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5-hmC, 5-mC & cytosine DNA standard pack
Description
The 5hmC, 5-mC & cytosine DNA standard pack includes hydroxymethylated, methylated and unmethylated DNA standards. These standards have been produced by PCR using the Diagenode’s MethylTaq DNA polymerase (Cat. No. AF-103-0250) and hm dCTP (Cat. No. AF-102-0300) in the case of the hydroxymethylated standard. The difference is that each contains either normal cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines.
| 5-hmC, 5-mC & cytosine DNA standard pack | AF-101-0002 | 2 µg | 290 $ |  |
Description
The 5-hmC, 5-mC & cytosine DNA standard pack includes hydroxymethylated , methylated and unmethylated DNA standards. The three provided DNA standards are linear dsDNA of ± 300 bp and have been produced by PCR using the Diagenode’s MethylTaq DNA polymerase (Cat. No. AF-103-0250) . The templates used for the PCR were DNA from Arabidopsis thaliana chromosome 1 (BAC F19K16) and chromosome 3 (BAC F24B22). The difference is that each contains either normal cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines. In the latter case, the Diagenode’s Hydroxymethyl dCTPs (Cat. No. AF-102-0300) have been used instead of normal cytosine nucleotides.
These methylation standards have been thoroughly tested in dot blot.
- Unmethylated control (300bp): [Chr3: 20074379-20074678]
- Methylated control (300bp): [Chr1: 30084349-30084050]
- Hydroxymethylated control (280 bp): [Chr1: 30003709-30003430]
Applications
Dot blot
Format
3 tubes each containing 2 µg of 100% unmodified cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine DNA standard
Storage
Store at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, and to prevent cross-contamination. Aliquoting is recommended.
Dot blot
