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Cell-O-Matic, an exciting program about single cell genomics

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Comparison of commercially available MeDIP kits

“…We observed that the MagMeDIP kit (Diagenode) had the highest yield…and the DNA was successfully enriched from a 1:4 to 10:1 ratio…”

H3K27me3 and H3K4me3

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Histone ChIP kit

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Description

Perform robust ChIP (Chromatin Immunoprecipitation) on 100.000 cells per IP. *Number of cells that can be lysed and sheared with this kit.

>> Check out our ChIP-Seq grade antibodies

Histone ChIP kit for 18 IPs

kch-orgHIS-012

18 rxns

270 $

Characteristics
Applications
Data sheets
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> Complete kit with all the reagents for your ChIP’ing needs (shearing buffers, control, primer pairs,…)
> Traditional method: 3 days from cell collection to PCR results (2 overnight incubations)
> Immunoprecipitation using agarose beads
> Includes control antibody directed against H3(K4me3)

The Histone ChIP kit has been validated using chromatin sheared by sonication using the Bioruptor®[Product Bioruptor]. The Kit provides you with eluted DNA ready to be purified with IPure[Product IPure]) or any other purification method of choice.

Chromatin Immunoprecipitation (ChIP)