Bioruptor® Standard

Special features

> Analog timer
> Metal soundproof box

Guarantee
Diagenode Instrument Limited Warranty Information.

Diagenode is committed to deliver superior product quality and performance, supported by industry-leading global service and technical support teams. Diagenode limited warranty for instruments and related components is set forth in the instrument user manual, in the limited warranty statement that accompanies the instrument when it is shipped, and in Diagenode' terms of sale. Generally, Diagenode warrants that all standard components of its instruments will be free of defects in materials and workmanship for a period of one (1) year from the date the warranty period begins, unless Diagenode' quotation or accompanying documentation states that a different warranty period or no warranty is provided. All warranty periods begin on the earlier of the date of installation. The instrument warranty is subject to certain exclusions, conditions, exceptions, and limitations. See those listed on request.

Please contact Diagenode if you have any questions about our warranties or would like information about post-warranty support.

Chromatin Shearing

DNA Shearing

Bacterial Cell Disruption

*Check our protocols and product information sections for the corresponding protocols or recommendations of use

Other applications:

• Dissolution of Aggregates
• Emulsification
• Homogenization
• Sonochemistry
• Cell Dispersion
• Mixing
• Synthesis
• Catalysis
• Microencapsulation
• Hydrolyzation
• Vaporization
• Nanoparticel Disaggregation

ChIP
Cuthbert G. L., Daujat S., Snowden A. W., Erdjument-Bromage H., Hagiwara T., Yamada, M., Schneider R., Gregory P.D., Tempst P., Bannister A.J., Kouzarides T. Histone deimination antagonizes arginine methylation. Cell, 118 (5), p.545-553 (2004).

Acevedo, LG, Iniguez, AL, Holster, HL, Zhang, X., Green, R., Farnham, PJ. Genome-scale ChIP-chip analysis using 10,000 human cells. Biotechniques. 43: 791-797 (2007).

Lopez-Serra L., Ballestar E., Fraga M.F, Alaminos M., Setien F. and Esteller M. A Profile of Methyl-CpG Binding Domain Protein Occupancy of Hypermethylated Promoter CpG Islands of Tumor Suppressor Genes in Human Cancer. Cancer Res 2006; 66: (17) (2006).

Sandmann T., Jakobsen J.S., Furlong, Eileen E.M. ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos. Nature protocols, 1 (6), p.2839-2855 (2006).

Navarro P., Page D.R., Avner P. and Rougeulle C. (2006). Tsix-mediated epigenetic switch of a CTCF-flanked region of the Xist promoter determines the Xist transcription program. GENES & DEVELOPMENT 20:2787-2792 (2006). Unité de Génétique Moléculaire Murine, Institut Pasteur 75724, Paris Cedex 15, France.

Durant M. and Pugh B.F. NuA4-Directed Chromatin Transactions throughout the Saccharomyces cerevisiae. Molecular and Cellular Biology, p. 5327–5335 (2007).

Mikkelsen T.S., Ku M., Jaffe D.B., Issac B., Lieberman E., Giannoukos G., Alvarez P., Brockman W., Kim T.K., Koche R.P., Lee W., Mendenhall E., O’Donovan A., Presser A., Russ C., Xie X., Meissner A., Wernig M., Jaenisch R., Nusbaum C., Lander E.S., Bernstein B.E. Genome-wide maps of chromatin state in pluripotent and lineage-committed cells. Nature; 448:553-560 (2007).

Rosenthal A.Z., Kim Y. and Gralla Y.D. Poising of Escherichia coli RNA Polymerase and Its Release from the sigma 38 C-Terminal Tail for osmY Transcription. J. Mol. Biol. 376, 938–949 (2008).

MeDIP
Yasui D.H., Peddada S., Bieda M.C., Vallero R.O., Hogart A., Nagarajan R.P., Thatcher K.N., Farnham P.J. and LaSalle J.M. Integrated epigenomic analyses of neuronal MeCP2 reveal a role for long-range interaction with active genes. 19416–19421 PNAS December 4, 2007 vol. 104 no. 49.

Library Preparation for next generation sequencing
Lister R., O’Malley R.C., Tonti-Filippini J., Gregory B.D., Berry C.C., A. Harvey Millar C.A. and Ecker J.R. Highly Integrated Single-Base Resolution Maps of the Epigenome in Arabidopsis. Cell 133, p523-536 (2008). Gregory B.D., O’Malley R.C.,Lister R., Urich M.A., Tonti-Filippini J., Chen H., Millar A.H. and Ecker J.R. A Link between RNA Metabolism and Silencing Affecting Arabidopsis Development. Developmental Cell 14, 854–866 (2008). Cokus S.J., Feng S., Zhang X., Chen Z., Merriman B., Haudenschild C.D., Pradhan S., Nelson S.F., Pellegrini M and Jacobsen S.E. Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning. Nature 452, 215-219 (2008).

Gregory B.D. Next generation sequencing library preparation following preparation of proper-sized DNA molecules using the Bioruptor® (complete application note http://www.diagenode.com/en/support/downloads/application.php).

Protein Extraction
Bunk V., Kaehne T., Degtyarev D., Shcherbakova T. and Reiser G. Novel isoenzyme of 2-oxoglutarate dehydrogenase is identified in brain, but not in heart. FEBS Journal 275, 4990-5006 (2008). S. Lee, V. Horn, E. Julien, Y. Liu, J. Wysocka, B. Bowerman, M. O. Hengartner, W. Herr. Epigenetic Regulation of Histone H3 Serine 10 Phosphorylation Status by HCF-1 Proteins in C. elegans and Mammalian Cells. PLoS ONE 2(11): e1213. doi:10.1371/journal.pone.0001213 (2007). Dissolution of aggregates Diehl F., Li M., He Y., Kinzler K. W., Vogelstein B., Dressman D. BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. Nature Methods, 3 (7), p.551-559 (2006).

Cell dispersion
Sun B., Tanji Y., Unno H. Influences of iron and humic acid on the growth of the cyanobacterium Anabaena circinalis. Biochemical Engineering Journal 24, 195–201 (2005).

Bacteria Lysis
Md. Kabir S., Yamashita D., Koyama S., Oshima T., Kurokawa K., Maeda M., Tsunedomi R., Murat M., Wada C., Mori H. and Yamada M. Cell lysis directed by σE in early stationary phase and effect of induction of the rpoE gene on global gene expression in Escherichia coli. Microbiology, 151, 2721–2735 (2005).