SX-8G IP-Star® Automated System

Major benefits

Ideal protocol optimization

•   Eliminates variation introduced by tools

•   Eliminates variation introduced by operator

•   Optimizes multiple parameters simultaneously

•   Accounts for complex interactions between experimental factors

Time saving

•   Improves time to result

•   Dramatically reduce hands-on time

•   Achieves flexible scheduling of experiments

Improves assay reproducibility

•   Increases consistency with automated assay processing

•   Achieves robust performance with validated reagents

Reduces contamination risk

Magnetic separation workflow

Magnetic beads are separated and captured on the inner wall of the pipette tip by a magnet that comes into contact with the tip. Liquid is released and the separated beads are moved to the next well. This technology allows for a cleaner assay with less carryover and background, differing from other systems that collect the bound DNA on the bottom of a reaction well.

	SX-8G IP-Star® Compact	SX-8G IP-Star®
Applications	ChIP-seq, MeDIP-seq, MethylCap-seq, IPure, Sample preparation, Re-ChIP, MagBisulfite, RNA-IP, Library preparation for NGS platforms.	ChIP-seq, MeDIP-seq, MethylCap-seq, IPure, Sample preparation, Re-ChIP, MagBisulfite, RNA-IP.
Software
User interface	Intuitive touch screen panel	PC Software
User friendly	Software training not required	Software training before use
Dispensing	Automated dispension of assay reagents	Manual dispension of assay reagents
Protocol optimization (flexible parameters)	Antibody coating (temperature, time, mixing speed) Immunoprecipitation (temperature, time, mixing speed) Washes (temperature, time, mixing speed)	Antibody coating (temperature, time) Immunoprecipitation (temperature, time)
New protocol development	Achievable by Diagenode product specialist	Achievable by customer after training
Characteristics	750W x 740 D x 610 H | 100 kg 8 Nozzles X-Y-Z axis | 4-95°C	1070W x 650 D x 780 H | 130 kg 8 Nozzles X-Y-Z axis | 4-95°C

© 2010. Diagnenode s.a. All rights reserved. IP-Star® is a registered trademark of Diagenode. IP-Star is a product range of consumables and reagents for Chromatin Immunoprecipitation and DNA Methylation studies. SX-8G is a Precision System Science Co., Ltd instrument. Diagenode is an exclusive distributor of SX-8G for North America and Europe for Epigenetic applications.

Chromatin Immunoprecipitation (ChIP)

Methylated DNA immunoprecipitation (MeDIP)

Methylbinding domain protein

O’Geen H, Echipare L, Farnham PJ.
Using ChIP-seq technology to generate high-resolution profiles of histone modifications.
Methods Mol Biol. 2011;791:265-86.

Sengenès J, Daunay A, Charles MA, Tost J.
Quality control and single nucleotide resolution analysis of methylated DNA immunoprecipitation products
Anal Biochem. 2010 Dec 1;407(1):141-3. Epub 2010 Jul 23.

Brinkman AB, Simmer F, Ma K, Kaan A, Zhu J, Stunnenberg HG.
Whole-genome DNA methylation profiling using MethylCap-seq.
Methods. 2010 Nov;52(3):232-6.Epub 2010 Jun 11.

Butcher LM, Beck S.
AutoMeDIP-seq: a high-throughput, whole genome, DNA methylation assay.
Methods. 2010 Nov;52(3):223-31. Epub 2010 Apr 10.

Bock C, Tomazou EM, Brinkman AB, Müller F, Simmer F, Gu H, Jäger N, Gnirke A, Stunnenberg HG, Meissner A.
Quantitative comparison of genome-wide DNA methylation mapping technologies.
Nat Biotechnol. 2010 Oct;28(10):1106-14. Epub 2010 Sep 19

Martens JH, Brinkman AB, Simmer F, Francoijs KJ, Nebbioso A, Ferrara F, Altucci L, Stunnenberg HG.
PML-RARalpha/RXR Alters the Epigenetic Landscape in Acute Promyelocytic Leukemia
Cancer Cell. 2010 Feb 17;17(2):173-85.