Bioruptor® in Action Video
SX-8G IP-Star® Compact Automated System
Chromatin immunoprecipitation and DNA methylation assays followed by next generation sequencing is a powerful technique for generating genome-wide profiles of epigenetic modifications and transcription factor binding sites. The SX-8G IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. Samples generated on the system are compatible with popular downstream applications such as QPCR, microarrays and next generation sequencing platforms. In addition to automated ChIP and DNA methylation protocols, this system contains a wide variety of protocols including next generation sequencing library preparation and sample purification.
|SX-8G IP-Star® Compact Automated System||B03000002 (UH-002-0001)||1 unit||CONTACT US|
- Easy-to-use user interface for protocol optimization
- Magnetic-bead technology for great reproducibility
- Reduced "hand on time" to just only 15 minutes
- Easy to use bench-top liquid handling system
- Process up to 16 samples in parallel
- True "walk-away" automation
- Flexibility to adapt your experimental conditions
Easy-to-use user interface based on an intuitive touch screen panel
ChIP-seq data produced using Diagenode' Auto Histone ChIP-seq kit and the IP-Star automated system
We performed in collaboration with the Genome Center (UC Davis) automated ChIP followed by next generation sequencing (Illumina Genome Analyzer) to map the epigenomic profiles of six major histone modifications in human primary T cells. >> See the poster
Figure 1. Auto ChIP-seq assays were performed on the SX8G IP-Star using primary human CD4 and CD8 T cells isolated by negative magnetic separation of peripheral blood mononuclear cells. Each Auto-ChIP sample was performed using Diagenode's Auto Histone ChIP-seq kit reagents and contained 1 ug of input chromatin. Illumina Genome Analyzer libraries were prepared and the samples were sequenced at the DNA Technologies Core at UC Davis.
Magnetic-bead technology for great reproducibility
© 2012. Diagnenode s.a. All rights reserved. IP-Star® is a registered trademark of Diagenode. IP-Star is a product range of consumables and reagents for Chromatin Immunoprecipitation and DNA Methylation studies. SX-8G is a Precision System Science Co., Ltd instrument. Diagenode is an exclusive distributor of SX-8G for North America and Europe for Epigenetic applications
- Sample preparation
- Library preparation for NGS platforms
O’Geen H, Echipare L, Farnham PJ.
Using ChIP-seq technology to generate high-resolution profiles of histone modifications.
Methods Mol Biol. 2011;791:265-86.
Sengenès J, Daunay A, Charles MA, Tost J.
Quality control and single nucleotide resolution analysis of methylated DNA immunoprecipitation products
Anal Biochem. 2010 Dec 1;407(1):141-3. Epub 2010 Jul 23.
Brinkman AB, Simmer F, Ma K, Kaan A, Zhu J, Stunnenberg HG.
Whole-genome DNA methylation profiling using MethylCap-seq.
Methods. 2010 Nov;52(3):232-6.Epub 2010 Jun 11.
Butcher LM, Beck S.
AutoMeDIP-seq: a high-throughput, whole genome, DNA methylation assay.
Methods. 2010 Nov;52(3):223-31. Epub 2010 Apr 10.
Bock C, Tomazou EM, Brinkman AB, Müller F, Simmer F, Gu H, Jäger N, Gnirke A, Stunnenberg HG, Meissner A.
Quantitative comparison of genome-wide DNA methylation mapping technologies.
Nat Biotechnol. 2010 Oct;28(10):1106-14. Epub 2010 Sep 19
Martens JH, Brinkman AB, Simmer F, Francoijs KJ, Nebbioso A, Ferrara F, Altucci L, Stunnenberg HG.
PML-RARalpha/RXR Alters the Epigenetic Landscape in Acute Promyelocytic Leukemia
Cancer Cell. 2010 Feb 17;17(2):173-85.