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SX-8G IP-Star® Compact Automated System
Description
Chromatin immunoprecipitation and DNA methylation assays followed by next generation sequencing is a powerful technique for generating genome-wide profiles of epigenetic modifications and transcription factor binding sites. The SX-8G IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. Samples generated on the system are compatible with popular downstream applications such as QPCR, microarrays and next generation sequencing platforms. In addition to automated ChIP [link to ChIP application page] and DNA methylation protocols [link to DNA methylation page], this system contains a wide variety of protocols including next generation sequencing library preparation and sample purification.
| SX-8G IP-Star® Compact Automated System | UH-002-0001 | 1 unit | CONTACT US | |
- Easy-to-use user interface for protocol optimization
- Magnetic-bead technology for great reproducibility
- Reduced «hand on time» to just only 15 minutes
- Easy to use bench-top liquid handling system
- Process up to 16 samples in parallel
- True "walk-away" automation
- Flexibility to adapt your experimental conditions
Easy-to-use user interface based on an intuitive touch screen panel
Results
ChIP-seq data produced using Diagenode' Auto Histone ChIP-seq kit and the IP-Star automated system
We performed in collaboration with the Genome Center (UC Davis) automated ChIP followed by next generation sequencing (Illumina Genome Analyzer) to map the epigenomic profiles of six major histone modifications in human primary T cells.
Figure 1. Auto ChIP-seq assays were performed on the SX8G IP-Star using primary human CD4 and CD8 T cells isolated by negative magnetic separation of peripheral blood mononuclear cells. Each Auto-ChIP sample was performed using Diagenode's Auto Histone ChIP-seq kit reagents and contained 1 ug of input chromatin. Illumina Genome Analyzer libraries were prepared and the samples were sequenced at the DNA Technologies Core at UC Davis.
Magnetic-bead technology for great reproducibility
© 2011. Diagnenode s.a. All rights reserved. IP-Star® is a registered trademark of Diagenode. IP-Star is a product range of consumables and reagents for Chromatin Immunoprecipitation and DNA Methylation studies. SX-8G is a Precision System Science Co., Ltd instrument. Diagenode is an exclusive distributor of SX-8G for North America and Europe for Epigenetic applications
- Sample preparation
- MeDIP
- hMeDIP
- MethylCap
- ChIP
- Re-ChIP
- MagBisulfite
- RNA-IP
- IPure
- Library preparation for NGS platforms
