Human TSH2B Primer Pairs

Description
The primer pair cat # pp-1041-050, -500 is specific to a CpG region of the TSH2B gene from human. The primers are optimized to be used in quantitative polymerase chain reaction (qPCR).

Application
The region amplified with TSH2B primer pair corresponds to genomic locus which is highly methylated in all somatic cells. The primers can be used as a positive control to amplify DNA isolated by Methylated DNA immunoprecipitation (MeDIP) or Methylated DNA Capture (meDNA capture) (Figure 3). In Chromatin Immunoprecipitation (ChIP) assay, the primers can be used as a control representing repressive chromatin.

Specificity
Human: positive
Other species: not tested

Lot #: 001

Concentration: 10 µM

Expected PCR product size: 170 base pairs (bp)

Amplified locus: chr6:25835060-25835229

Format
In solution in MilliQ water at the concentration of 10 µM (each oligonucleotide primer is at the final concentration of 5 µM).

Storage
For long storage, store at -20°C. Avoid multiple freeze-thaw cycles.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.