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H3K36me3 monoclonal antibody - Classic
Monoclonal antibody raised in mouse against histone H3 trimethylated at lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide (1).
| H3K36me3 monoclonal antibody - Classic | MAb-183-050 | 50 μg/ 50 μl | 295 $ |  |
Specificity
Human: positive
Other species: not tested
Applications
ChIP (1-5 μg/ChIP)
ELISA (1:3,000)
Dot blotting (1:10,000)
Western blotting (1:1,000 - 1:2,000)
Immunofluoresence (1:500)
Lot #: 001-11
Concentration: 1 µg/µl
Source: Mouse
Type: Monoclonal ChIP-grade
IgG isotype: IgG1
Purity
Protein A purified monoclonal antibody in PBS containing 0.05% azide.
Storage
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Application key
ChIP-seq = Chromatin Immunoprecipitation sequencing; ChIP = Chromatin Immunoprecipitation; hMeDIP-seq = Hydroxymethylated DNA Immunoprecipitation sequencing; hMeDIP = Hydroxymethylated DNA Immunoprecipitation; MeDIP-seq = Methylated DNA Immunoprecipitation sequencing; MeDIP = Methylated DNA Immunoprecipitation; WB = Western blot; EIA/ELISA = Enzyme immunoassay/ Enzyme-linked immunosorbent assay; EMSA = Electrophoretic Mobility Shift Assay; FACS = Flow Cytometry; IF = Immunofluorescence; IHC = Immunohistochemistry; FACS/Flow cytometry; IS = Immunostaining
ChIP - ELISA - Dot blot - WB - IF
(1) Kimura H, Hayashi-Takanaka Y, Goto Y, Takizawa N and Nozaki N (2008) The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies. Cell Struct Funct 33: 61-73.
(2) Rechtsteiner A, Ercan S, Takasaki T, Phippen TM, Egelhofer TA, Wang W, Kimura H, Lieb JD and Strome S (2010) The Histone H3K36 Methyltransferase MES-4 Acts Epigenetically to Transmit the Memory of Germline Gene Expression to Progeny. PLoS Genet. 6: e1001091.
(3) Luco RF, Pan Q, Tominaga K, Blencowe BJ, Pereira-Smith OM and Misteli T (2010) Regulation of Alternative Splicing by Histone Modifications. Science 327: 996–1000.
(4) Furuhashi H, Takasaki T, Rechtsteiner A, Li T, Kimura H, Checchi PM, Strome S and Kelly WG (2010) Transgenerational epigenetic regulation of C. elegans primordial germ cells. Epigenetics Chromatin 3: 15.
