H3K27me3 polyclonal antibody

H3K27me3 is silencing expression very efficiently. Trimethylation of lysine 27 on H3 has been strongly related to transcriptional repression in numerous important processes in animals and plants such as during development and disease. This modification is maintained by Polycomb Repressive Complex 2 (catalyzed by EZH2 methyltransferase) and can be taken out by lysine demethylases like JMJD3.

Specificity
Human: positive
Other species: not tested

Applications
ChIP/ChIP-seq (1 μg/ChIP)
ELISA (1:1,000)
Dot blotting (1:10,000)
Western blotting (1:1,00)

Lot #: A1456-004P

Concentration: 1.04 μg/μl

Source: Rabbit

Type: Polyclonal

Purity
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.

Storage
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Application key
ChIP-seq = Chromatin Immunoprecipitation sequencing; ChIP = Chromatin Immunoprecipitation; hMeDIP-seq = Hydroxymethylated DNA Immunoprecipitation sequencing; hMeDIP = Hydroxymethylated DNA Immunoprecipitation; MeDIP-seq = Methylated DNA Immunoprecipitation sequencing; MeDIP = Methylated DNA Immunoprecipitation; WB = Western blot; EIA/ELISA = Enzyme immunoassay/ Enzyme-linked immunosorbent assay; EMSA = Electrophoretic Mobility Shift Assay; FACS = Flow Cytometry; IF = Immunofluorescence; IHC = Immunohistochemistry; FACS/Flow cytometry

ChIP/ChIP-seq
ELISA - Dot blot - WB