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5-mC monoclonal antibody 33D3 - Premium

The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. It has been validated for Methylated DNA Immunoprecipitation (MeDIP-seq, MeDIP-on-chip), Immunofluorescence, Flow Cytometry, Dot blot & ELISA.

Diagenode is the exclusive worldwide source of genuine 33D3 clone!


5-mC monoclonal antibody 33D3 - Premium C15200081-10 (MAb-081-010) 10 µg/5 µl 80 $
5-mC monoclonal antibody 33D3 - Premium C15200081-100 (MAb-081-100) 100 µg/50 µl 460 $
5-mC monoclonal antibody 33D3 - Premium C15200081-500 (MAb-081-500) 500 µg/250 µl 1600 $

Specificity
Human, mouse: positive
Other species: not tested

Applications
MeDIP (1-2 µg per IP)
ELISA (1:100)
Dot blotting (1:250)
Immunofluorescence (1:500)
FISH (1:500)
Southern blot (1:200)


Lot #: GF-003

Concentration: 2 µg/µl

Source: Mouse

Type: Monoclonal MeDIP-grade
Isotype: IgG1

Purity
Monoclonal antibody purified by gel filtration, in PBS containing 0.05% azide.

Storage
Store in small aliquots at -80°C. Avoid multiple freeze-taw cycles.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

Application key
ChIP-seq = Chromatin Immunoprecipitation sequencing; ChIP = Chromatin Immunoprecipitation; hMeDIP-seq = Hydroxymethylated DNA Immunoprecipitation sequencing; hMeDIP = Hydroxymethylated DNA Immunoprecipitation; MeDIP-seq = Methylated DNA Immunoprecipitation sequencing; MeDIP = Methylated DNA Immunoprecipitation; WB = Western blot; EIA/ELISA = Enzyme immunoassay/ Enzyme-linked immunosorbent assay; EMSA = Electrophoretic Mobility Shift Assay; FACS = Flow Cytometry; IF = Immunofluorescence; IHC = Immunohistochemistry; FACS/Flow cytometry; IS = Immunostaining


MeDIP - ELISA - Dot blot - IF - FISH - Southern blot