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5-mC monoclonal antibody 33D3 - Premium
The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. It has been validated for Methylated DNA Immunoprecipitation (MeDIP-seq, MeDIP-on-chip), Immunofluorescence, Flow Cytometry, Dot blot & ELISA.
Diagenode is the exclusive source of genuine 33D3 clone!
| 5-mC monoclonal antibody 33D3 - Premium | MAb-081-010 | 10 µg/5 µl | 70 $ |  |
| 5-mC monoclonal antibody 33D3 - Premium | MAb-081-100 | 100 µg/50 µl | 460 $ | |
| 5-mC monoclonal antibody 33D3 - Premium | MAb-081-500 | 500 µg/250 µl | 1600 $ | |
Specificity
Human, mouse: positive
Other species: not tested
Applications
MeDIP (1-2 µg per IP)
ELISA (1:100)
Dot blotting (1:250)
Immunofluorescence (1:500)
FISH (1:500)
Southern blot (1:200)
Lot #: GF-002
Concentration: 2 µg/µl
Source: Mouse
Type: Monoclonal MeDIP-grade IgG1
Purity
Monoclonal antibody purified by gel filtration, in PBS containing 0.05% azide.
Storage
Store in small aliquots at -80°C. Avoid multiple freeze-taw cycles.
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Application key
ChIP-seq = Chromatin Immunoprecipitation sequencing; ChIP = Chromatin Immunoprecipitation; hMeDIP-seq = Hydroxymethylated DNA Immunoprecipitation sequencing; hMeDIP = Hydroxymethylated DNA Immunoprecipitation; MeDIP-seq = Methylated DNA Immunoprecipitation sequencing; MeDIP = Methylated DNA Immunoprecipitation; WB = Western blot; EIA/ELISA = Enzyme immunoassay/ Enzyme-linked immunosorbent assay; EMSA = Electrophoretic Mobility Shift Assay; FACS = Flow Cytometry; IF = Immunofluorescence; IHC = Immunohistochemistry; FACS/Flow cytometry; IS = Immunostaining
MeDIP - ELISA - Dot blot - IF - FISH - Southern blot
