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H3K27ac polyclonal antibody - Classic

Polyclonal antibody raised in rabbit against histone H3, acetylated at lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.


H3K27ac polyclonal antibody - Classic pAb-174-050 50 μg/ 42 μl 295 $

Specificity
Human: positive
Other species: not tested

Applications
ChIP/ChIP-seq (1-2 μg per ChIP)
ELISA (1:100)
Dot blotting (1:25,000)
Western blotting (1:1,000)

Lot #: A7071-001P

Concentration: 1.2 μg/μl

Source: Rabbit

Type: Polyclonal ChIP-grade/ChIP-seq-grade

Purity
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.

Storage
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.

Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

Application key
ChIP-seq = Chromatin Immunoprecipitation sequencing; ChIP = Chromatin Immunoprecipitation; hMeDIP-seq = Hydroxymethylated DNA Immunoprecipitation sequencing; hMeDIP = Hydroxymethylated DNA Immunoprecipitation; MeDIP-seq = Methylated DNA Immunoprecipitation sequencing; MeDIP = Methylated DNA Immunoprecipitation; WB = Western blot; EIA/ELISA = Enzyme immunoassay/ Enzyme-linked immunosorbent assay; EMSA = Electrophoretic Mobility Shift Assay; FACS = Flow Cytometry; IF = Immunofluorescence; IHC = Immunohistochemistry; FACS/Flow cytometry; IS = Immunostaining


ChIP/ChIP-seq
ELISA - Dot blot - WB


(1) Kimura H, Hayashi-Takanaka Y, Goto Y, Takizawa N and Nozaki N (2008) The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies. Cell Struct Funct 33: 61-73.