Chromatin shearing

High powered ultrasound waves can produce gaseous cavitation (see figure 1) in liquids.

Cavitation is the formation of small bubbles of dissolved gases or vapors due to the alteration of pressure in liquids. These bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming.

This mechanical stress has multiple effects on biological samples including; effective cell lysis, DNA and chromatin shearing as well as homogenization.

Diagenode’s Bioruptor Sonicator is the ideal tool for reproducible chromatin shearing (figure 2) and has acquired an unmatched reputation in the scientific community.

Figure 2: Chromatin has been sheared using the Bioruptor Sonicator. The sheared chromatin is analyzed on a 1% agarose gel. Chromatin shearing efficiency is analyzed alongside the DNA molecular weight marker in the left lane.

Diagenode’s Bioruptor® sonication device:

STANDARD		PLUS		TWIN		XL
Bioruptor® (UCD-200)		Bioruptor® Plus (UCD-300)		Bioruptor® Twin (UCD-400)		Bioruptor® XL (UCD-500)
Chromatin Shearing DNA Shearing Cell Lysis		Chromatin Shearing DNA Shearing Cell Lysis		Chromatin Shearing DNA Shearing Cell Lysis		Cell Lysis DNA Shearing
Standard, basic model with reliable performance across applications		Upgrade of UCD-200 with improved displays and controls for greater timing accuracy and temperature control		Provides high-throughput ability and processes up to 24 samples simultaneously		Provides high-throughput ability and processes up to 48 samples simultaneously
Tube size	# of tubes processed	Tube size	# of tubes processed	Tube size	# of tubes processed	Tube size	# of tubes processed
0.5 ml 1.5 ml 10 ml 15 ml 50 ml	12 6 6 6 3	0.5 ml 1.5 ml 10 ml 15 ml 50 ml	12 6 6 6 3	0.5 ml 1.5 ml 10 ml 15 ml 50 ml	24 12 12 12 6	0.5 ml 1.5 ml 10 ml 15 ml 50 ml	48 24 24 24 6
Analog timer		Digital timer & controls		Digital timer & controls		Digital timer & controls
Metal soundproof box		Metal soundproof box		Metal soundproof box		Metal soundproof box
		Control system for regulated water flow between Bioruptor® and Bioruptor® Water Cooler (BioAcc-cool). Includes overheat shutdown protection.		Control system for regulated water flow between Bioruptor® and Bioruptor® Water Cooler (BioAcc-cool). Includes overheat shutdown protection.
TPX Microtubes Bioruptor® 0.5 ml Microtubes for DNA Shearing		TPX Microtubes Bioruptor® 0.5 ml Microtubes for DNA Shearing		TPX Microtubes Bioruptor® 0.5 ml Microtubes for DNA Shearing		TPX Microtubes Bioruptor® 0.5 ml Microtubes for DNA Shearing