Applications | Chromatin shearing

Chromatin shearing

High powered ultrasound waves can produce gaseous cavitation (see figure 1) in liquids.

Cavitation is the formation of small bubbles of dissolved gases or vapors due to the alteration of pressure in liquids. These bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming.

This mechanical stress has multiple effects on biological samples including; effective cell lysis, DNA and chromatin shearing as well as homogenization.

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Diagenode’s Bioruptor Sonicator is the ideal tool for reproducible chromatin shearing (figure 2) and has acquired an unmatched reputation in the scientific community.

Figure 2: Chromatin has been sheared using the Bioruptor Sonicator. The sheared chromatin is analyzed on a 1% agarose gel. Chromatin shearing efficiency is analyzed alongside the DNA molecular weight marker in the left lane.

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Diagenode’s Bioruptor® sonication device:

STANDARD	STANDARD	HIGH THROUGHPUT	HIGH THROUGHPUT
Bioruptor® (UCD-200)	Bioruptor® Next Gen (UCD-300)	Bioruptor® Twin (UCD-400)	Bioruptor® XL (UCD-500)
Number of tubes to be processed simultaneously: 12 (0.5 ml); 6 (1.5 ml); 6 (15 ml); 3 (50 ml) Analog timer Plastic (or metal uprade) soundproof box	Number of tubes to be processed simultaneously: 12 (0.5 ml); 6 (1.5 ml); 6 (15 ml); 3 (50 ml) Digital timer Metal soundproof box Overheat shutdown protection Possibility to control cooling system Machine lifetime control	Number of tubes to be processed simultaneously: 24 (0.5 ml); 12 (1.5 ml); 12 (15 ml); 6 (50 ml) Digital timer Metal soundproof box Overheat shutdown protection Possibility to control cooling system Machine lifetime control	Number of tubes to be processed simultaneously: 48 (0.5 ml); 24 (1.5 ml); 24 (15 ml); 12 (50 ml) Digital timer Metal soundproof box Ideal for cell lysis. Not validated for all kind of chromatin samples.
Validated for ChIP, MeDIP, MBD & Shotgun libraries (sequencing)
Compatible with the SX-8G IP-Star Automated System for ChIP, MeDIP & MBD

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