Applications | Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Kits

Diagenode offers a wide range of kits for Chromatin Immunoprecipitation, a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. The ChIP assay offers great potential to improve knowledge about the regulation of gene expression. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.

>> Technical overview

>> Learn more about Chromatin Function


 

Manual ChIP and ChIP-seq kits

Automated ChIP and ChIP-seq kits

 

Manual ChIP and ChIP-seq kits

Need some guidance?
  Number of cells required per IP Recommended applications
True MicroChIP kit +
MicroPlex Library Preparation kit (1)
10,000 (2) to 100,000 ChIP-seq or qPCR
iDeal ChIP-seq kit +
MicroPlex Library Preparation kit (1)
100,000 to 1,000,000 ChIP-seq or qPCR
LowCell# ChIP kit 1,000 (3) to 1,000,000 qPCR
HighCell# ChIP kit 1,000,000 to 10,000,000 qPCR

  (1) Optional for ChIP-seq
  (2) Chromatin Shearing on 10,000 cells
  (3) Chromatin Shearing on 100,000 cells

 

Automated ChIP and ChIP-seq kits

Need some guidance?
  Number of cells required per IP Recommended applications
Auto True MicroChIP kit +
MicroPlex Library Preparation kit (1)
10,000 (2) to 100,000 ChIP-seq or qPCR
Auto Histone ChIP-seq kit +
MicroPlex Library Preparation kit (1)
> 100,000 ChIP-seq or qPCR
Auto Transcription ChIP kit +
MicroPlex Library Preparation kit(1)
> 100,000 ChIP-seq or qPCR

  (1) Optional for ChIP-seq
  (2) Chromatin Shearing on 10,000 cells

 

Technical overview

  • Add magnetic beads coated with antibody of interest
  • Magnetic capture of Antibody - Protein - DNA complex
  • Washes
  • Reverse cross-linking and DNA purification DNA is ready for further analysis (qPCR, amplification, microarray, sequencing)