Applications | Chromatin Immunoprecipitation (ChIP)

Chromatin Immunoprecipitation (ChIP)

Diagenode offers a wide range of kits for Chromatin Immunoprecipitation, a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. The ChIP assay offers great potential to improve knowledge about the regulation of gene expression. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.

How it works

Chromatin is first treated with formaldehyde to fix the chromatin-bound proteins to the DNA, followed by shearing into small fragment sizes (200bp – 1kb). This step is followed by immunoprecipitation using a specific ChIP-grade antibody. Crosslinking is reversed followed by proteinase K treatment or DNA purification using magnetic beads (IPure kit). The purified DNA is analyzed to identify the genomic regions where the specific protein was located (next generation sequencing, qPCR, hybridization on microarray).

Overview