True MicroChIP kit

• Revolutionary: Highly efficient ChIP recovery and enrichment from only 10,000 cells
• Reliable and accurate sequencing library preparation from just picogram inputs
• Validated on studies for histone marks

A: ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10.000 cells and amplification of 35 pg of DNA ChIP'd from 100.000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina® Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.
B: We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

iDeal ChIP-seq kit

• Validated: Only kit on the market validated for GAIIx (Illumina®) and PGM™ (Ion Torrent™)
• Proven: Our expertise with ChIP-seq tools enables reproducible and efficient results every time
• Complete: Kit includes control antibodies, control primer pairs and IPure DNA purification module

Fig. A shows a several hundred bp stretch along the 12th chromosome, where the high similarity of read distribution can be observed, despite the radically different characteristics of the sequencers. ChIP was performed on chromatin from HelaS3 cells using 1 µg of H3K4me3 antibody.
Fig. B is a close capture focusing on the well-known GAPDH gene. The detailed view reveals that even the peak structure is similar.

LowCell# ChIP kit

The only ChIP kit that can be used with as low as 200 cells per IP.

• Use from 200 - 1,000,000 cells per IP
• Ideal for stem cells, embryos and biopsies
• Improved handling and reproducibility due to magnetic beads and magnetic rack (DiaMag02)
• Optimized reverse-crosslinking step

LowCell# ChIP kit allows for ChIP with 1,000 to 1,000,000 cells per IP. Diagenode antibody directed against H3K4me3 and an optimized qPCR primer pair to amplify a region of the c-fos promoter were used. ChIP has been performed using 1,000, 10,000, 100,000 NCCIT cells and 1 million U20S cells. 1 µg of antibody was used per ChIP experiment. A negative control antibody was included in the ChIP assay (1µg/IP).

HighCell# ChIP kit

Perform ChIP with up to 10 million cells per IP. Recover more DNA and limit bias of downstream applications.

• Use from 1,000,000 - 10,000,000 cells per IP
• Recover large amounts of DNA
• Limit bias of possible downstream amplification steps (e.g. prior to next generation sequencing)
• Ideal for ChIP of low abundant proteins
• Improved handling and reproducibility due to magnetic beads and a uniquely designed magnetic rack (DiaMag1.5)

HighCell# ChIP kit permits high recovery of DNA with Pol II antibody and 5 million U20S. 6.5 µl of the Poll antibody has been used per IP. C-fos has been used as positive locus and myoglobin exon 2 as negative locus. The DNA recovered after ChIP was 170 ng per IP.

Transcription ChIP kit

Complete solution for ChIP on transcription factors.

• Complete kit with all the reagents for your ChIP needs (shearing buffers, control, primer pairs)
• Includes Control antibody directed against TBP (TATA binding protein)
• Standard method: 3 days from cell collection to PCR results
• Immunoprecipitation using agarose beads

ChIP results obtained with the Diagenode Transcription ChIP kit including the TBP antibody. ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP and optimized PCR primer pairs for qPCR.

OneDay ChIP kit

Designed to perform ChIP assays in abundance.

• Protocol requires only 1 or 2 days from cell collection to your results (qPCR)
• Optimized purification and decrosslinking step
• Validated with our shearing ChIP Kits (Cat. No. kch-redmod-100, kch-shchro-040)
• Immunoprecipitation using agarose beads

ChIP results obtained with the Diagenode OneDay ChIP kit. ChIP assays were performed using hepatoma HTC-IR cells and antibodies directed against RNA polymerase II. Cells were non-induced and induced with 0.1 µM PMA (phorbol 12-myristate 13-acetate) for 10 and 30 minutes as indicated in the graph. Sheared chromatin was obtained with an "in house" protocol (see the "starting material" and "additional protocols" sections). Optimized qPCR primer pairs to amplify the promoter of the PMA-inducible egr gene were used.

Histone ChIP kit

Complete solution for ChIP on histones and their modifications.

• Complete kit with all the reagents for your ChIP needs (shearing buffers, control, primer pairs)
• Includes control antibody directed against H3K4me3
• Standard method: 3 days from cell collection to PCR results
• Immunoprecipitation using agarose beads

ChIP results obtained with the Diagenode Histone ChIP kit. Occupancy of the c-fos and b-actin promoters by modified histones is evident based on fluorescent qPCR analysis of immunoprecipitated DNA. Controls for IP and PCR specificity include primers for the myoglobin exon 2 and BMX (18 kb downstream of txn start).

Auto Histone ChIP-seq kit

• Use from 1,000-5,000,000 cells per IP
• Validated for SX-8G IP-Star® Automated System
• Validated in ChIP-seq experiments with antibodies directed to histone modifications
• validated in ChIP-on- ChIP experiments with antibodies directed to histone modifications

Auto ChIP-seq assays were performed on the SX-8G IP-Star® using primary human CD4 and CD8 T cells isolated by negative magnetic separation of peripheral blood mononuclear cells. Each Auto-ChIP sample was performed using Diagenode's Auto Histone ChIP-seq kit reagents and contained 1 ug of input chromatin. Illumina Genome Analyzer libraries were prepared and the samples were sequenced at the DNA Technologies Core at UC Davis.

Auto Transcription ChIP kit

• Use from 100,000-10,000,000 cells per IP
• Validated for SX-8G IP-Star® Automated System
• Validated in ChIP experiments with antibodies directed to transcription factors and epitope tagged transcription factors
• Validated in ChIP-on ChIP experiments with antibodies directed to histone modifications

Auto ChIP experiment was performed using the H3K27me3, Pol II and TBP antibodies and the Auto Transcription ChIP kit. Sheared chromatin from 2.5 x 10^6 U2OS cells was used in each ChIP reaction. Results are graphed as percentage of the input. C-fos and GADPH promoter regions were tested as positive binding sites for pol II and TBP whereas the exon 2 of Myoglobin was tested as negative control region for both transcription factors. H3K27me3 was used as antibody control for the ChIP experiment

Auto ChIP kit

Designed to perform automated ChIP using the SX-8G IP-Star® Automated System enabling highly reproducible results and allowing for high throughput.

• Use from 1,000-5,000,000 cells per IP
• Validated on SX-8G IP-Star® which eliminates variation introduced by tools and operators
• Decreases hands on time

Efficiency in the recovery for the positive locus SAT2 whereas. ChIP assays was performed using the SX-8G IP Star automated system. Diagenode antibodies directed against against H3K9me3 (Cat. No. pAb-056-050) as well as optimized qPCR primers to amplify a region of the c-fos promoter (Cat. No. pp-1004-050, -500) and SAT2 from the IP'd DNA were also used. Chromatin was sheared from 100,000 cells. Per ChIP experiment: 10,000 cells equivalent and 1 ug of antibody were used. A negative control antibody (negative IgG from rabbit: 1 ug/IP) and no antibody control were included in the ChIP assay.

• Add magnetic beads coated with antibody of interest
• Magnetic capture of Antibody - Protein - DNA complex
• Washes
• Reverse cross-linking and DNA purification DNA is ready for further analysis (qPCR, amplification, microarray, sequencing)