Various biochemical and analytical techniques require the extraction of protein and/or RNA from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry, or protein purification. Isolating intact RNA is essential for many techniques used in gene expression analysis.

In order to obtain optimal yields of proteins and RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diagenode's Bioruptor® uses state-of-the-art ultrasound ACT (Adaptive Cavitation Technology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA and protein extraction. Bioruptor® offers unique benefits for cell lysis and tissue homogenization:

	Bioruptor® Sonicator	Competitor C Sonicator	Tip/Probe Sonicators
Multiplexing capability		NO	NO
High-throughput		NO	NO
Simple operation
Consistency between samples within runs			NO
Cost of operation	Low	High	Low
Value	High	Low	Low
Protein Preservation	High	High	Low

* Comparison made between the Bioruptor®, Competitor C and Tip/Probe sonicators

Bioruptor® Plus

RNA extraction kit

Protein extraction kit & Protein extraction beads

1.5 ml TPX microtubes

15 ml TPX tubes