Bioruptor® in Action Video
Cell & Tissue disruption workflow
Applications | Cell & Tissue disruption
Various biochemical and analytical techniques require the extraction of protein and/or RNA from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry, or protein purification. Isolating intact RNA is essential for many techniques used in gene expression analysis.
Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).
Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).
- Bioruptor® Standard or Plus
- Bioruptor® Water Cooler
- Single Cycle Valve (required for Bioruptor® Plus)
- 15 ml sonication accessories for Bioruptor® Standard & Plus
- RNA extraction kit
In order to obtain optimal yields of proteins and RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diagenode's Bioruptor® uses state-of-the-art ultrasound ACT (Adaptive Cavitation Technology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA and protein extraction. Bioruptor® offers unique benefits for cell lysis and tissue homogenization:
|Bioruptor® Sonicator||Competitor C Sonicator||Tip/Probe Sonicators|
|Consistency between samples within runs||NO|
|Cost of operation||Low||High||Low|
Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico | Download
Protein Extraction from Tissues and Cultured Cells using Bioruptor® (Standard/Plus) | Download
RNA Extraction from tissue using Bioruptor® (Standard/Plus) | Download
Bacteria Cell Disruption using Bioruptor® | Download
|>> Learn more about specifications of the different Bioruptor® models.
>> See all applications possible with Bioruptor® technology.