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 HMGA1 Reprograms Somatic Cells into Pluripotent Stem Cells 
 
The researchers in this study revealed a novel role for the high mobility group A1 (HMGA1) gene as a key regulator of the stem cell state. HMGA1 enhances cellular reprogramming from a somatic cell to a fully pluripotent stem cell along with several key pluripotency genes such as SOX2 and cMYC. HMGA1 binds to the promoters of these genes suggesting that it directly induces their expression. HMGA1 also promotes an undifferentiated, pluripotent state and blocks differentiation. Although additional studies are needed, the findings suggest that HMGA1 transcriptional networks are important in reprogramming normal cells into stem-like, malignant cancer cells and that these pathways could be targeted in therapy. Several Diagenode tools - the Bioruptor®, Auto-histone ChIP-seq kit, and SX-8G IP-Star® Compact Automated System were used to help gain results for the study.
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 Ash2 stabilizes the histone methyltransferase Trr 
 
Ecdysone triggers chromatin changes via histone modifications required for gene regulation. When activated, the ecdysone receptor binds to a domain that contains histone H3 methyltransferase trithorax-related protein (Trr). Methylation of histone H3K4me, which is associated with transcriptional activation, requires cofactors, including Ash2. Mutants lacking ash2 have severe defects because ecdysone-responsive genes are not activated. This lack of transcriptional activation is due to the absence of H3K4me3 marks set by Trr. Thus, they proposed that Ash2 functions together with Trr as an ecdysone receptor coactivator. The Diagenode antibody anti-H3K4me1 was used to help obtain results in the study.
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